Engineering human renal epithelial cells for transplantation in regenerative medicine
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Keywords
Three-dimensional culture
Cell encapsulation
Conformal coating
Hydrogel
Gene therapy
Trophic factors
Abbreviations
2D
Two-dimensional
3D
Three-dimensional
ALG
alginate
CCE
conformal coating encapsulation
DTT
dithiothreitol
dVS
divinyl sulfone
EGF
epidermal growth factor
FGFb
basic fibroblast growth factor
GFP
green fluorescent protein
GM-CSF
granulocyte macrophage colony-stimulating factor
HBSS
Hanks' balanced salt solution
HGF
hepatocyte growth factor
hRECs
Human Renal Epithelial Cells
MCP-1
monocyte chemotactic protein-1 (CCL2)
MMP-2
matrix metalloproteinases-2
MOI
multiplicity of infection
MTT
3-(4,5-Dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide
PAI-1
plasminogen activator inhibitor-1
PBS
phosphate-buffered saline
PEG
polyethylene glycol
pHEMA
Poly(2-hydroxyethyl methacrylate)
PPG
polypropylene glycol
RPM
revolutions per minute
SCF
stem cell factor
TEA
triethanolamine
TGFα
transforming growth factor alpha
TIMP
tissue inhibitor of metalloproteinase
UP-MVG
ultrapure medium viscosity (> 200 mPas) sodium alginate where minimum 60% of the monomer units are guluronate
VCAM-1
vascular cell adhesion molecule-1
VEGF
vascular endothelial growth factor
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© 2017 The Author(s). Published by Elsevier Ltd on behalf of IPEM.