BioDome regenerative sleeve for biochemical and biophysical stimulation of tissue regeneration

Abstract 

Previous research on vertebrate limb regeneration indicates there are several mediating factors involved during the re-growth process. These factors are both biochemical and biophysical. While the phenomenon of adult limb regeneration does not occur naturally in mammalian species, prior research has focused mainly on biochemical modes of stimulating tissue growth and regeneration. The BioDome was aimed at developing a new experimental tool to permit the more systematic study of the impact of biophysical and biochemical factors on mammalian tissue regeneration. The BioDome is a multi-component sleeve assembly that encompasses the wound site of an amputated murine digit and provides an environment conducive to tissue regeneration. The studies showed that the BioDome was effective in supporting early stages of murine digit tip regeneration when combined with a porcine urinary bladder matrix (UBM) pepsin digest and electrical stimulation. The hydrated inner environment of the BioDome influenced regeneration, with additional effects seen with the application of electrical stimulation and pharmacological treatments.

Abbreviations: ECM, extracellular matrix, EGF, epidermal growth factor, GAG, glycosaminoglycan, HP-EGF, heparin-binding epidermal growth factor, LMEC, large mononuclear eosinophilic cell, MMP, matrix metalloproteinase, TGF-α, transforming growth factor α, UBM, (porcine) urinary bladder matrix

Keywords: Tissue regeneration, BioDome, Biophysical, Electrical stimulation