Medical Engineering & Physics
Volume 28, Issue 5 , Pages 468-474 , June 2006

An in line non-invasive optical system to monitor pH in cell and tissue culture

  • Xia Xu

      Affiliations

    • Department of Engineering Science, University of Oxford, Parks Rd., Oxford OX1 3PJ, UK
    • ,
  • Stanton Smith

      Affiliations

    • University Laboratory of Physiology, University of Oxford, Parks Rd., Oxford OX1 3PT, UK
    • ,
  • Jill Urban

      Affiliations

    • University Laboratory of Physiology, University of Oxford, Parks Rd., Oxford OX1 3PT, UK
    • ,
  • Zhanfeng Cui

      Affiliations

    • Department of Engineering Science, University of Oxford, Parks Rd., Oxford OX1 3PJ, UK
    • Corresponding Author InformationCorresponding author. Tel.: +44 1865 273118; fax: +44 1865 283273.

Received 2 March 2005 ,Revised 1 July 2005 ,Accepted 11 July 2005.

References 

  1. Razaq S, Wilkins RJ, Urban JPG. The effect of extracellular pH on matrix turnover by cells of the bovine nucleus pulposus. Eur Spine J. 2003;12:341–349
  2. Smith A, Pollard M, Cleaton-Jones P, Preston A. A comparison of three electrodes for the measurement of pH in small volumes. Caries Res. 1997;31:55–59
  3. Ahn BK, Liu CC, Neuman MR, Ko WK. Development of a miniature pH electrode for biological applications. Biomed Sci Instrum. 1971;8:81–82
  4. Salkind AJ, Imran M, Mackenzie JM, Shapiro B. Improving intramuscular pH needle electrode stability. Med Instrum. 1981;15:126–127
  5. Jayasundar R, Hall LD, Bleehen NM. Comparison of pH measurements made using 31P NMR and a fibreoptic pH meter. NMR Biomed. 1992;5:360–363
  6. Jeevarajan AS, Vani S, Taylor TD, Anderson MM. Continuous pH monitoring in a perfused bioreactor system using an optical pH sensor. Biotech Bioeng. 2002;78:467–472
  7. Hwang EY, Pappas O, Jeevarajan AS, Anderson MM. Evaluation of the paratrend multi-analyte sensor for potential utilization in long-duration automated cell culture monitoring. Biomed Microdevices. 2004;6:241–249
  8. Manz A, Graber N, Widmer MH. Miniaturised total chemical analysis systems: a novel concept for chemical sensing. Sens Actuators. 1990;B1:244–248
  9. Freaney R, McShane A, Keaveny TV, McKenna M, Rabenstein K. Novel instrument for real-time monitoring using miniaturized flow system with integrated biosensors. Ann Clin Biochem. 1997;34:291–302
  10. Webster JR, Burns MA, Burke DT, Mastrangelo CH. Monolithic capillary electrophoresis device with integrated fluorescence detector. Anal Chem. 2001;73:1622–1626
  11. Hübner J, Mogensen KB, Jorgensen AM, Friis P, Telleman P, Kutter JP. Intregated optical measurement system for fluorescence spectroscopy in microfluidic channels. Rev Sci Instrum. 2001;72:229–233
  12. Schabmueller CGJ, Pollard JR, Evans AGR, Wilkinson JS, Ensel Gl, Brunnschweiler A. Integrated diode detector and optical fibres for in situ detection within micromachined polymerase chain reaction chips. J Micromech Microeng. 2001;11:329–333
  13. Camou S, et al. Design of a 2-D optical lens on a PDMS micro-chip to improve fluorescence spectroscopy using integrated optical fibres. In: Proceedings of MOEMS 2001. Okinawa, Japan. 2001;p. 133–134
  14. Papadopoulos N, Limniou M, Koklamanis G, Tsarouxas A, Roilidis M, Bigger SW. Spec UV–Vis: an ultraviolet–visible spectrophotometer simulation. J Che Educ. 2001;78:1515–1560
  15. Putman RW. Intracellular pH regulation. In:  Sperelakis N editors. Cell physiology source book. New York: Academic press; 1995;
  16. Kragh-Hansen U, Möller JV. Protein binding of small molecules II. Role of electrostatic forces for binding of phenol red by human serum albumin. Biochim Biophys Acta. 1973;295:447–456
  17. Besa SSA, Kelly SW, Greenhalgh PA. Simple fiber optic spectrophotometric cell for pH determination. J Biomed Eng. 1989;11:151–156

PII: S1350-4533(05)00155-4

doi: 10.1016/j.medengphy.2005.07.016

Medical Engineering & Physics
Volume 28, Issue 5 , Pages 468-474 , June 2006

Article Tools

* Image Usage & Resolution