An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging

Martin, Matt E.; Wabuyele, Musundi; Panjehpour, Masoud; Overholt, Bergein; DeNovo, Robert; Kennel, Steve; Cunningham, Glenn; Vo-Dinh, Tuan

doi:10.1016/j.medengphy.2005.04.022

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Volume 28, Issue 2 , Pages 149-155, March 2006

An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging

Matt E. Martin
- Center for Advanced Biomedical Photonics, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6101, USA
- Tennessee Technological University, Mechanical Engineering Department, Cookeville, TN 38501, USA
Musundi Wabuyele
- Center for Advanced Biomedical Photonics, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6101, USA
Masoud Panjehpour
- Thompson Cancer Center, 1915 White Ave., Knoxville, TN 37916, USA
Bergein Overholt
- Thompson Cancer Center, 1915 White Ave., Knoxville, TN 37916, USA
Robert DeNovo
- Department of Small Animal Clinical Sciences, University of Tennessee Veterinary School, Knoxville, TN 37996, USA
Steve Kennel
- Center for Mammalian Genetics, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
Glenn Cunningham
- Tennessee Technological University, Mechanical Engineering Department, Cookeville, TN 38501, USA
Tuan Vo-Dinh
- Center for Advanced Biomedical Photonics, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6101, USA
- Corresponding author. Tel.: +1 865 574 6249.

Received 16 August 2004; received in revised form 18 March 2005; accepted 12 April 2005. published online 16 June 2005.

Abstract

An acousto-optic tunable filter (AOTF)-based system for dual-modality hyperspectral imaging (DMHSI) has been developed for use in characterization of normal and malignant mouse tissue. The system consists of a laser, endoscope, AOTF, and two cameras coupled with optics and electronics. Initial results show that the system can delineate normal and malignant mouse tissues real-time. The analysis shows that malignant tissues consistently exhibit less fluorescent intensity in the wavelength band from 440 to 540nm with a peak intensity of around 490nm. The analysis also shows key spectroscopic differences between normal and malignant tissues. Further, these results are compared to real-time spectroscopic data and show good correlation.